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An Introduction to Poliovirus: Pathogenesis, Vaccination, and the Endgame for Global Eradication
Pages 1-10
This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
An Introduction to Poliovirus: Pathogenesis, Vaccination, and the Endgame for Global Eradication
Pages 1-10
Poliovirus Laboratory Based Surveillance: An Overview
Pages 11-18
Isolation and Characterization of Enteroviruses from Clinical Samples
Pages 19-28
Isolation and Characterization of Poliovirus in Cell Culture Systems
Pages 29-53
Molecular Characterization of Polio from Environmental Samples: ISSP, The Israeli Sewage Surveillance Protocol
Pages 55-107
Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays
Pages 109-127
A Transgenic Mouse Model of Poliomyelitis
Pages 129-144
Standardized Methods for Detection of Poliovirus Antibodies
Pages 145-176
Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing by PCR and Real-Time RT-PCR
Pages 177-212
Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative
Pages 213-226
Phylogenetic Analysis of Poliovirus Sequences
Pages 227-237
Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA
Pages 239-249
A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses
Pages 251-262
Methods to Monitor Molecular Consistency of Oral Polio Vaccine
Pages 263-277
Methods for the Quality Control of Inactivated Poliovirus Vaccines
Pages 279-297
Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays
Pages 299-323
Identification and Analysis of Antiviral Compounds Against Poliovirus
Pages 325-338
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