Titolo: Light Microscopy

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Radiologia - Diagnostica per Immagini

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Light Microscopy

Methods and Protocols

Editore

Springer

Anno

2017

Pagine

285

ISBN

9781493968084

I prezzi indicati possono subire variazioni poiché soggetti all'oscillazione dei cambi delle valute e/o agli aggiornamenti effettuati dagli Editori.

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This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. Light Microscopy: Methods and Protocols puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Light Microscopy: Methods and Protocols offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in routine practices. This book also serves as a useful teaching manual in practical courses on light microscopy.

Introduction to Modern Methods in Light Microscopy

Ryan, Joel (et al.)

Pages 1-15
Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)

Pampaloni, Francesco (et al.)

Pages 19-31
Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy

Jährling, Nina (et al.)

Pages 33-49
Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis

Grinsven, Erinke (et al.)

Pages 51-71
Imaging of Brain Slices with a Genetically Encoded Voltage Indicator

Quicke, Peter (et al.)

Pages 73-84

FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells

Kraft, Axel E. (et al.)

Pages 85-90
Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum

Domozych, David (et al.)

Pages 91-105
Targeted Ablation Using Laser Nanosurgery

Gayathri Vegesna, Naga Venkata (et al.)

Pages 107-125
Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells

Bach, Juri N. (et al.)

Pages 129-141
STED Imaging in Drosophila Brain Slices

Fendl, Sandra (et al.)

Pages 143-150
Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells

Trexler, Adam J. (et al.)

Pages 151-165
Optical Coherence Microscopy

Leitgeb, Rainer A.

Pages 167-182
Designing Image Analysis Pipelines in Light Microscopy: A Rational Approach

Arganda-Carreras, Ignacio (et al.)

Pages 185-207
Automated Analysis of Intracellular Dynamic Processes

Yao, Yao (et al.)

Pages 209-228
Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells

Rino, José (et al.)

Pages 229-242
Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP)

Giakoumakis, Nickolaos Nikiforos (et al.)

Pages 243-267
Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening

Gunkel, Manuel (et al.)

Pages 269-280