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Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection
Pages 3-24
Punto di Riferimento
per Medici e Operatori Sanitari
per Medici e Operatori Sanitari
LIBRERIA STUDIUM
Human T-Lymphotropic Viruses
Methods and Protocols
Casoli
Editore
Springer
Anno
2017
Pagine
220
ISBN
9781493968701
90,00 €
DA SCONTARE
While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens. The contents include essential aspects of epidemiology and virus transmission, novel and robust methodologies for studying the effects of trans-activating regulatory HTLVs’ proteins, the latest techniques for genotyping and gene expression analysis, as well as cellular phenotype and dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Human T-Lymphotropic Viruses: Methods and Protocols serves as an ideal guide to an area of study that is very much worthy of further research.
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Molecular Epidemiology Database for Sequence Management and Data Mining
Pages 25-31
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Reporter Systems to Study HTLV-1 Transmission
Pages 33-46
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Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells
Pages 47-55
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Isolation of Exosomes from HTLV-Infected Cells
Pages 57-75
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A Luciferase Functional Quantitative Assay for Measuring NF-ĸB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins
Pages 79-87
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Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2
Pages 89-101
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EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway
Pages 103-108
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Methods for Identifying and Examining HTLV-1 HBZ Post-translational Modifications
Pages 111-126
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High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites
Pages 127-141
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STR Profiling of HTLV-1-Infected Cell Lines
Pages 143-154
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Expression of HTLV-1 Genes in T-Cells Using RNA Electroporation
Pages 155-170
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Quantification of Cell Turnover in the Bovine Leukemia Virus Model
Pages 173-182
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Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection
Pages 183-194
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Overview of Targeted Therapies for Adult T-Cell Leukemia/Lymphoma
Pages 197-216
