Contents

Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
PART I
1 Gross Examination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Stephen A. Geller and Richard E. Horowitz
2 The Pathologist’s Guide to Fixatives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Kiran Qidwai, Michelle Afkhami, and Christina E. Day
3 Tissue Processing and Hematoxylin and Eosin Staining . . . . . . . . . . . . . . . . . . 31
Ada T. Feldman and Delia Wolfe
4 Light Microscope Alignment Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Robert Bagnell
5 Immunohistochemistry in Surgical Pathology: Principles and Practice . . . . . . . 81
Clive R. Taylor
6 Optimizing Direct Immunofluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Ian D. Odell and Deborah Cook
7 Electron Microscopy: A Brief History and Review of Current
Clinical Application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Ronald E. Gordon
8 A Practical Approach to Clinical and Research Biobanking . . . . . . . . . . . . . . . 137
William H. Yong, Sarah M. Dry, and Maryam Shabihkhani
PART II
9 Hospital Autopsy Performance and Reporting. . . . . . . . . . . . . . . . . . . . . . . . . 165
Jacqueline Benjamin
10 Characterization of HER2 Status by Fluorescence In Situ
Hybridization (FISH) and Immunohistochemistry (IHC) . . . . . . . . . . . . . . . . 181
Oliver A. Press, Roberta Guzman, Monica Cervantes,
Angela Santiago, and Michael F. Press
11 Intraoperative Frozen Section Consultation in Gynecologic Pathology . . . . . . 209
Fabiola Medeiros and Juan C. Felix
12 Processing Dermatological Biopsies. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221
Gene Kim, Brittney DeClerck, and Eric Sorenson
13 Human Papillomavirus Testing in Head and Neck Squamous
Cell Carcinoma: Best Practice for Diagnosis . . . . . . . . . . . . . . . . . . . . . . . . . . 237
Andrew Schache, James Croud, Max Robinson,
and Selvam Thavaraj

14 Peripheral Blood Smears, Bone Marrow Aspiration, Trephine
and Clot Biopsies: Methods and Protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
Michelle Afkhami, Maria Vergara-Lluri, Russell K. Brynes,
and Imran N. Siddiqi
15 Processing the Lymph Node Biopsy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Eric Loo and Imran N. Siddiqi
16 Handling and Interpretation of Heart Transplant Biopsies. . . . . . . . . . . . . . . . 283
Eric Swanson and W. Dean Wallace
17 Handling and Interpretation of Lung Transplant Biopsies . . . . . . . . . . . . . . . . 293
Eric Swanson and W. Dean Wallace
18 Liver: Tissue Handling and Evaluation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303
Stephen A. Geller
19 Handling and Interpretation of the Medical Renal Biopsy . . . . . . . . . . . . . . . . 323
Michael N. Koss
20 Optimizing Prostate Specimen Handling for Diagnosis and Prognosis . . . . . . . 337
David G. Bostwick, Christina E. Day, and Isabelle Meiers
21 Optimal Handling of Bone Tumor Specimens . . . . . . . . . . . . . . . . . . . . . . . . . 353
Leslie G. Dodd, Shi Wei, and Gene P. Siegal
22 CNS Intraoperative Consultation: A Survival Guide
for Non-neuropathologists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 369
Jesse Lee Kresak, Marie Rivera-Zengotita, Robin M. Foss,
and Anthony T. Yachnis
23 Examining and Processing Eye Specimens . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377
Amir A. Azari, Nasreen A. Syed, and Daniel M. Albert
24 Optimizing the Skeletal Muscle Biopsy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397
Karen M. Weidenheim
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 411