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Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture
Pages 3-24
Punto di Riferimento
per Medici e Operatori Sanitari
per Medici e Operatori Sanitari
LIBRERIA STUDIUM
High-Resolution Imaging of Cellular Proteins
Methods and Protocols
Schwartzbach, Skalli, Schikorski
Editore
Springer
Anno
2016
Pagine
366
ISBN
9781493963508
110,00 €
DA SCONTARE
This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.
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Antibody Production with Synthetic Peptides
Pages 25-47
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Production and Purification of Polyclonal Antibodies
Pages 49-59
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Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab′)
Pages 61-71
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Helper-Dependent Adenoviral Vectors and Their Use for Neuroscience Applications
Pages 73-90
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Localizing Proteins in Fixed
Pages 93-111
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Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells
Pages 113-123
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Using FRAP or FRAPA to Visualize the Movement of Fluorescently Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses
Pages 125-151
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Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein–Protein Interactions and Assessment of Subcellular Localization in Live Cells
Pages 153-170
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Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging
Pages 171-185
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Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH-Sensitive Fluorescent Proteins
Pages 187-200
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Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas
Pages 203-216
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Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells
Pages 217-232
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Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues
Pages 233-242
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Immunoelectron Microscopy of Cryofixed Freeze-Substituted Yeast
Pages 243-258
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Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes
Pages 259-267
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Pre-embedding Nanogold Silver and Gold Intensification
Pages 269-278
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Post-embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based on Heat-Induced Antigen Retrieval
Pages 279-290
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Pre- and Post-embedding Immunogold Labeling of Tissue Sections
Pages 291-307
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Immunogold Labeling for Scanning Electron Microscopy
Pages 309-325
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Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope
Pages 327-341
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Functional Nanoscale Imaging of Synaptic Vesicle Cycling with Superfast Fixation
Pages 343-358
