Titolo: Herpes Simplex Virus

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Immunologia e Allergologia

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Herpes Simplex Virus

Methods and Protocols

Editore

Springer

Anno

2020

Pagine

457

ISBN

9781493998135

I prezzi indicati possono subire variazioni poiché soggetti all'oscillazione dei cambi delle valute e/o agli aggiornamenti effettuati dagli Editori.

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This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV.

Tour de Herpes: Cycling Through the Life and Biology of HSV-1

Pages 1-30

Denes, Christopher E. (et al.)
Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology

Pages 31-56

Sandgren, Kerrie J. (et al.)
Herpes Simplex Virus Growth, Preparation, and Assay

Pages 57-72

Sutter, Sereina O. (et al.)
Engineering HSV-1 Vectors for Gene Therapy

Pages 73-90

Goins, William F. (et al.)
Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors

Pages 91-109

Fraefel, Cornel (et al.)

HSV-1 Amplicon Vectors as Genetic Vaccines

Pages 111-130

Meier, Anita F. (et al.)
oHSV Genome Editing by Means of galK Recombineering

Pages 131-151

Menotti, Laura (et al.)
Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein

Pages 153-168

Vannini, Andrea (et al.)
CRISPR/Cas9-Based Genome Editing of HSV

Pages 169-183

Velusamy, Thilaga (et al.)
Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescence In Situ Hybridization (FISH)

Pages 185-197

Cohen, Camille (et al.)
Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for Use in High-Throughput Sequencing

Pages 199-217

Shipley, Mackenzie M. (et al.)
HSV Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo

Pages 219-239

Sawtell, Nancy M. (et al.)
Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals

Pages 241-261

Sauerbrei, Andreas (et al.)
Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation

Pages 263-277

Hu, Hui-Lan (et al.)
Characterization of Extracellular HSV-1 Virions by Proteomics

Pages 279-288

Lippé, Roger
Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry

Pages 289-303

Khadivjam, Bita (et al.)
Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells

Pages 305-317

Bello-Morales, Raquel (et al.)
Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot

Pages 319-326

Sari, Tri Komala (et al.)
BioID Combined with Mass Spectrometry to Study Herpesvirus Protein–Protein Interaction Networks

Pages 327-341

Cheerathodi, Mujeeb R. (et al.)
Preparation of Herpes Simplex Virus-Infected Primary Neurons for Transmission Electron Microscopy

Pages 343-354

Miranda-Saksena, Monica (et al.)
Transmission Immunoelectron Microscopy of Herpes Simplex Virus-1-Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane

Pages 355-364

Miranda-Saksena, Monica (et al.)
Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication

Pages 365-376

Seyffert, Michael (et al.)
Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination

Pages 377-393

White, Ellen M. (et al.)
Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination

Pages 395-407

Cooper, Rebecca S. (et al.)
The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1

Pages 409-418

Danastas, Kevin (et al.)

A Model of In Vivo HSV-1 DNA Transport Using Murine Retinal Ganglion Cells

Pages 419-428

LaVail, Jennifer H.
The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines

Pages 429-454

Marshak, Joshua O. (et al.)