Titolo: Hepatitis B Virus

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Hepatitis B Virus

Methods and Protocols

Editore

Springer

Anno

2017

Pagine

297

ISBN

9781493966981

I prezzi indicati possono subire variazioni poiché soggetti all'oscillazione dei cambi delle valute e/o agli aggiornamenti effettuati dagli Editori.

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This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Hepatitis B Virus: Methods and Protocols is a valuable tool for researchers to use toward their advanced studies in HBV.

NTCP-Reconstituted In Vitro HBV Infection System

Sun, Yinyan (et al.)

Pages 1-14
Hepatitis B Virus Infection of HepaRG Cells, HepaRG-hNTCP Cells, and Primary Human Hepatocytes

Ni, Yi (et al.)

Pages 15-25
Live Cell Imaging Confocal Microscopy Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells

König, Alexander (et al.)

Pages 27-36
Intracytoplasmic Transport of Hepatitis B Virus Capsids

Osseman, Quentin (et al.)

Pages 37-51
A Homokaryon Assay for Nucleocytoplasmic Shuttling Activity of HBV Core Protein

Yang, Ching-Chun (et al.)

Pages 53-58

Analyses of HBV cccDNA Quantification and Modification

Xia, Yuchen (et al.)

Pages 59-72
Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation-Specific PCR

Zhang, Yongmei (et al.)

Pages 73-84
A T7 Endonuclease I Assay to Detect Talen-Mediated Targeted Mutation of HBV cccDNA

Bloom, Kristie (et al.)

Pages 85-95
Detection of Hepatocyte Clones Containing Integrated Hepatitis B Virus DNA Using Inverse Nested PCR

Tu, Thomas (et al.)

Pages 97-118
Highly Sensitive Detection of HBV RNA in Liver Tissue by In Situ Hybridization

Calabrese, Diego (et al.)

Pages 119-134
Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice

Allweiss, Lena (et al.)

Pages 135-142
Measuring Changes in Cytosolic Calcium Levels in HBV- and HBx-Expressing Cultured Primary Hepatocytes

Casciano, Jessica C. (et al.)

Pages 143-155
In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase

Clark, Daniel N. (et al.)

Pages 157-177
In Vitro Enzymatic and Cell Culture-Based Assays for Measuring Activity of HBV RNaseH Inhibitors

Lomonosova, Elena (et al.)

Pages 179-192
Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay

Yan, Ran (et al.)

Pages 193-202
Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications

Goddard, Cally D. (et al.)

Pages 203-209
Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform

Long, Quan-Xin (et al.)

Pages 211-218
Generation of Replication-Competent Hepatitis B Virus Genome from Blood Samples for Functional Characterization

Qin, Yanli (et al.)

Pages 219-226
Hydrodynamic HBV Transfection Mouse Model

Wu, Li-Ling (et al.)

Pages 227-235
An ELISPOT-Based Assay to Measure HBV-Specific CD8+ T Cell Responses in Immunocompetent Mice

Reynolds, Tracy D. (et al.)

Pages 237-247
Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells, and Kupffer Cells

Liu, Jia (et al.)

Pages 249-258
Partial Hepatectomy and Castration of HBV Transgenic Mice

Tian, Yongjun (et al.)

Pages 259-266
Studying HBV Infection and Therapy in Immune-Deficient NOD-Rag1−/−IL2RgammaC-null (NRG) Fumarylacetoacetate Hydrolase (Fah) Knockout Mice Transplanted with Human Hepatocytes

Li, Feng (et al.)

Pages 267-276
Measurement of Antiviral Effect and Innate Immune Response During Treatment of Primary Woodchuck Hepatocytes

Murreddu, Marta G. (et al.)

Pages 277-294