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The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc.
The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing.
Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique.
The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.
Front Matter
Pages 1-1
Fixation of Histology Samples: Principles, Methods and Types of Fixatives
Pages 3-18
Processing of Tissue in the Histopathology Laboratory
Pages 19-28
Embedding of Tissue in Histopathology
Pages 29-34
Decalcification of Bony and Hard Tissue for Histopathology Processing
Pages 35-40
Tissue Microtomy: Principle and Procedure
Pages 41-50
Frozen Section: Principle and Procedure
Pages 51-56
Staining Principle and General Procedure of Staining the Tissue
Pages 57-70
Haematoxylin and Eosin Stain of the Tissue Section
Pages 71-82
Special Stains for the Carbohydrate, Protein, Lipid, Nucleic Acid and Pigments
Pages 83-99
Connective Tissue Stain: Principle and Procedure
Pages 101-111
Pages 113-115
Stains for the Microbial Organisms
Pages 117-122
Front Matter
Pages 123-123
Cytology Sample Procurement, Fixation and Processing
Pages 125-136
Routine Staining in Cytology Laboratory
Pages 137-141
The Basic Technique of Fine Needle Aspiration Cytology
Pages 143-150
Front Matter
Pages 151-151
Immunocytochemistry in Histology and Cytology
Pages 153-177
Flow Cytometry: Basic Principles, Procedure, and Applications in Pathology
Pages 179-194
Pages 195-203
Automation in the Laboratory and Liquid-Based Cytology
Pages 205-214
Polymerase Chain Reaction: Principle, Technique and Applications in Pathology
Pages 215-228
Fluorescent In Situ Hybridisation Techniques in Pathology: Principle, Technique and Applications
Pages 229-239
Tissue Microarray in Pathology: Principal, Technique and Applications
Pages 241-246
Pages 247-261
Liquid Biopsy: Basic Principles, Techniques and Applications
Pages 263-266
Artificial Neural Network in Pathology: Basic Principles and Applications
Pages 267-275
Front Matter
Pages 277-277
Compound Light Microscope and Other Different Microscopes
Pages 279-288
Pages 289-301
Electron Microscopy: Principle, Components, Optics and Specimen Processing
Pages 303-313
Quality Control and Laboratory Organization
Pages 315-322
Laboratory Safety and Laboratory Waste Disposal
Pages 323-328
Pages 329-340
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