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A Practical Guide to Frozen Section Technique
Peters
Editore
Springer
Anno
2010
Pagine
192
ISBN
9781441912336
75,00 €
I prezzi indicati possono subire variazioni poiché soggetti all'oscillazione dei cambi delle valute e/o agli aggiornamenti effettuati dagli Editori.
  • Presents detailed descriptions of techniques used in Mohs surgery
  • Features original techniques garnered from experience and teaching
  • Highly illustrated handbook ideal for onsite use

A Practical Guide to Frozen Section Technique offers an easy to learn approach to frozen section technique in the form of a highly illustrated handbook intended for onsite use in the laboratory. The book begins with a novel, clearly delineated, step by step approach to learning continuous motion brush technique. Emphasis is placed on recognizing and correcting artifacts during the preparation process. The book addresses all of the steps in the preparation of slides from cutting through cover-slipping. The author’s unique, original techniques for tissue embedding including face down embedding in steel well bars, frozen block cryoembedding and paper cryoembedding are detailed. Variables key to the quality of the preparation including block temperature, tissue properties and section thickness are detailed. The book also covers understanding the cryostat and basic maintenance and care.Sections covering techniques used in Mohs dermatologic surgery, and techniques used in basic animal and human research are discussed by noted experts in their field.

A Practical Guide to Frozen Section Technique will be of great value to pathologists, pathology residents in training and also experimental pathology researchers that rely upon this methodology to perform tissue analysis in research.

ix
Contents
1 Understanding and Maintaining the Cryostat........................................ 1
Philip Hyam
2 Gross Examination of Tissues in the Frozen Section Room................... 13
Stephen R. Peters
3 Embedding of Tissue for Frozen Section................................................. 37
Stephen R. Peters
4 Cutting the Frozen Section........................................................................ 75
Stephen R. Peters
5 Variables Affecting the Cutting Properties of Tissues
and the Resulting Artifacts....................................................................... 97
Stephen R. Peters
6 Fixation, Staining and Coverslipping of Frozen Section Slides............. 117
Stephen R. Peters and Catherine Susan Delia
7 Microscopic Interpretation in the Frozen Section Setting..................... 131
Stephen R. Peters
8 Frozen Section Techniques Used in Mohs
Micrographic Surgery............................................................................... 151
Barbara Beck and Stephen R. Peters
9 Frozen Section Technique in the Animal Research Setting................... 171
Charles W. Scouten
Index.................................................................................................................. 191
 

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